Direct Dynamic Protein-Affinity Selection Mass-Spectrometry
نویسندگان
چکیده
منابع مشابه
Direct Dynamic Protein-Affinity Selection Mass-Spectrometry
A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERalpha-LBD). In-solution incubation is performed of the analyte and the His-tagged ERalpha-LBD. The bound complex is immobilized on a nickel-loaded protein-affinity selection column, where after the unbound fraction is removed. The immobilized pro...
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A precise and rapid method for identifying sites of interaction between proteins was demonstrated; the basis of the method is direct mass spectrometric readout from the complex to determine the specific components of the proteins that interact--a method termed affinity-directed mass spectrometry. The strategy was used to define the region of interaction of a protein growth factor with a monoclo...
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The recent sequencing of the human genome has revealed that the human cell contains only twice the number of genes as the worm or fly (1). This implies that the regulation of gene products as well as their interactions accounts for the increased biological complexity of higher organisms. Consequently to exploit the wealth of information provided by genome sequencing it is essential to be able t...
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Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identification of individual gel bands. However, todays mass spectrometers are highly sensitive, and powe...
متن کاملFocus on affinity mass spectrometry.
A lthough the application of affinity techniques in the analysis of biopolymers by mass spectrom-etry has become an established approach over the last decade or so, it is re-emerging as new methodology combinations and tools for the structure identification and quantification of biopolymer interactions become available. It can be productively used for the identification of proteins and protein ...
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ژورنال
عنوان ژورنال: Chromatographia
سال: 2010
ISSN: 0009-5893,1612-1112
DOI: 10.1365/s10337-010-1586-x